A comparison of methods measuring nitrogen fixation in a laboratory setting
Name of Award
3M Small Scale Grant
Jill Welter, Associate Professor of Biology, and Jackelyn Goldschmidt, student researcher, received a $2,000 award from the 3M Small Scale Grant program to assess the effectiveness of current methods for quantifying N2-fixation, as well as how different methods vary as a function of temperature. They will measure N2-fixation using three methods that are currently used in the field: acetylene reduction assay, 15N2 biomass incorporation method, and 15N2 gas consumption in the water, in a lab setting to see how the methods compare across a temperature gradient (5°C, 10°C, 15°C, and 20°C). The goal is to better understand how each of these methods responds to temperature, in particular the ARA method and the 15N2 biomass incorporation method, and to see if the 15N2 gas method is able to answer those questions by providing more exact measurements.
Welter, Jill PhD and Goldschmidt, Jackelyn, "A comparison of methods measuring nitrogen fixation in a laboratory setting" (2014). Internal Grant Awards. 134.