Development of a Flow Cytometry Method for Analysis of DNA Ploidy in Lumbriculus

Name of Award

3M Small Scale Grant

Date Awarded




Project Description

Kay Tweeten, Professor of Biology, and Samantha Morris, student researcher, received a $2,000 award from the 3M Small Scale Grant program to develop a flow cytometry method for analysis of ploidy levels in various populations of Lumbriculus. The number of chromosomes in these annelids is currently determined using time-consuming and inefficient methods such as chromosome spreads or chromosome splash techniques. There is need for a rapid, more reliable protocol that can be applied in ploidy analysis of worms collected from natural habitats.

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